N lipids required for RC biogenesis. This hypothesis is consistent with our finding that the requirement for GBF1 is strongest early in DENV infection, and disappears as infection progresses (i.e. after RCs have formed). We also note that ZW10, a validated interactor of NS3 whose knockdown inhibited DENV replication by 70 (Fig. 3), is also involved in trafficking between the ER and the Golgi (77)